Transplanting live mushroom tissue to a pre-poured agar plate is a useful way to create a pure culture from a live mushroom. This can be done to isolate a particular strain or to propagate more mycelium for future use. The process needs to be performed with great care to avoid contamination.

Materials Needed:

- Myco Junkies Pre Poured Sterile Agar Plate
- Live mushroom specimen
- Sterile scalpel or blade
- Sterile gloves
- 70% Isopropyl alcohol (for sterilization)
- Still Air Box (SAB) or Glove Box (optional but highly recommended)
- Face mask
- Clean workspace

Instructions:

1. Prepare Your Workspace: Clean your workspace thoroughly, including any surfaces and tools you'll use. You can use a solution of 1 part bleach to 10 parts water, or a commercial antibacterial cleaner.

2. Prepare Your Still Air Box (SAB) or Glove Box: If you have one, set up your SAB or glove box. This helps provide a clean environment with minimal air movement, reducing the risk of contamination. Clean the interior of the box and the gloves attached to it with 70% isopropyl alcohol.

3. Personal Hygiene: Wash your hands and wear a face mask. Put on your sterile gloves.

4. Prepare the Mushroom: Choose a healthy, mature mushroom. Wipe the outside of the mushroom with a paper towel soaked in 70% isopropyl alcohol. Let it air dry for a few moments.

5. Sterilize the Scalpel: Sterilize your scalpel or blade by flaming it until it glows red hot, then allow it to cool. Do not touch the sterilized blade to any non-sterile surfaces.

6. Extract Mushroom Tissue: Carefully cut open the mushroom, exposing the inner tissue. This tissue is generally more protected from contaminants than the outer surfaces. With your sterilized scalpel, cut a small piece of the inner tissue.

7. Transfer Tissue to Agar Plate: Quickly open the agar plate just enough to slip the scalpel inside and gently place the tissue on the agar. Try to minimize the time the plate is open to reduce contamination risk.

8. Seal and Store the Agar Plate: Once the tissue is on the agar, seal the plate with parafilm or micropore tape to prevent contamination.

9. Incubate: Place the agar plate in a warm, dark place (around 75-80°F or 24-27°C). After a few days to a week, you should see mycelium beginning to grow out from the tissue onto the agar.

10. Monitor Growth: Keep an eye on the plate for any signs of contamination (molds of different colors, odd smells, etc.). If the plate becomes contaminated, it should be discarded.

11. Expand the Culture: Once the mycelium covers the plate, you can use it to inoculate grain jars, spawn bags, or more agar plates.

Remember, cleanliness is crucial in this process. Any contamination on the scalpel, mushroom, or your hands can ruin the agar plate and your mycelium culture. Always work as sterile as possible.